Glutathione reductase, glutathione peroxidase, and catalase in rats exposed to mercury after the addition of ir bagendit rice leaf water infusion

Abstract

Objective: The study objective was to determine the antioxidant levels of GSr, GPx, and CAT after adding rice leaf infusion to mice exposed to mercury.

Methods: Research method: Randomised post-test only control-group design in groups of experimental animals from male Rattus norvegicus according to inclusion and exclusion criteria. ANOVA, Kruskal-Wallis, and Bonferroni's Post Hoc statistical tests were used to test the differences between each group. The experimental animals consisted of 5 groups: negative control group, positive control group, and treatments 1, 2 and 3. Each group consisted of 8 experimental animals, totalling 40 experimental animals. The positive control group was only exposed to mercury. In contrast, the treatment group, apart from being exposed to mercury, was also exposed to IR Bagendit rice leaf infusion with various doses. The desired intervention was tried for 15 days. Examine GSr, GPx, and CAT using an experimental animal serum with the ELISA method.

Results: The results showed that the average levels of GSr, GPx, and CAT were higher in all treatment and negative control groups compared to the positive control group. Statistically, there was a significant difference in GPx (p=0.03) and CAT (0.02) levels. Furthermore, post hoc analysis using the Bonferroni test obtained significant differences in GPx and CAT levels between the positive and negative control groups and treatment 3.

Conclusion: In conclusion, there was an increase in antioxidant levels in the treatment group after adding water and IR Bagendit rice infusion.