Protective Role of Resveratrol Against Isoproterenol-induced Myocardial Infarction in Male Rats

Abstract

Objective: The present study aimed to evaluate the protective effect of resveratrol against isoproterenol-induced myocardial infarction in male Sprague-Dawley rats.

Methods: The study included 40 rats aged 3 to 4 months, weighing 200 to 250 grams, randomly distributed into four groups. The first group (Control) received 1 ml of distilled water. The second group (Resveratrol) received 20 mg/kg orally at a dose of 2 ml daily. The third group (Isoproterenol) received 100 mg/kg intraperitoneally (IP). The fourth group, Res + ISO, received oral resveratrol (20 mg/kg/day) for 21 days. After resveratrol, they received IP injections of 100 mg/kg of ISO on days 20 and 21 of the resveratrol regimen for 21 days.

Results: The results showed that isoproterenol caused a significant increase in markers of oxidative stress (MDA), inflammation (TNF-α), triglyceride levels, cholesterol, and cardiac enzymes (CK-MB, cTnI, LDH), along with a decrease in total antioxidant activity (T-AOC). In contrast, treatment with resveratrol resulted in a significant improvement in these markers, as elevated values ​​decreased and antioxidant levels returned to normal, indicating the effect of resveratrol in reducing oxidative and inflammatory damage. The group treated with both compounds showed intermediate results, demonstrating a clear protective role of resveratrol against the toxic effects of isoproterenol.

Conclusion: These findings suggest that resveratrol is a potential substance for avoiding cardiomyopathy and enhancing heart function in situations of oxidative and inflammatory stress since it has anti-hyperlipidemia, antioxidant, and anti-inflammatory qualities.